Conversely, the essential oil of anise in which the percentage of monoterpenes was as low as 2. All the essential oils showed antioxidant activity. Several methods have been developed to assess the radical scavenging activity. Free radical scavenging and antibacterial activities of helichrysum caespititium dc harv. The measurement of the dpph radical scavenging activity was performed according to methodology described by brandwilliams et al. The control solution was prepared by mixing ethanol 3. For determination of radical scavenging activity of different foods, beverages and substrates were elaborated a. Dec 09, 2008 the objective of the present study was to evaluate the antioxidant potential and free radical scavenging activity of a 70% methanol extract of s. In our present study the highest antioxidant and radical scavenging activities of the tanacetum parthenium extracts and parthenolide in terms of their free radical scavenging activities have been studied. Hydroxyl radical scavenging activity the hydroxyl radical scavenging activity was determined according to the method of beara et al.
Standardized methods for the determination of antioxidant. Comparison between the radical scavenging activity and. Superoxide radical o2 was generated from the photoreduction of riboflavin and was deducted by nitro blue tetrazolium dye nbt reduction method. Dpph free radical scavenging assay11 dpph 1,1diphenyl2picrylhydrazyl radical scavenging assay is a stable free radical with purple color, the intensity of which is measured at 510 nm spectrophotometrically. Statistical analysis the experiment was done in triplicate for each substance. We compared the hydroxyl radical scavenging activity of 25 antioxidant samples prepared in ethanol solution with samples prepared after removing the ethanol residue. Hydrogen peroxide assay catalog number de3700 for the quantitative determination of hydrogen peroxide h2o2 concentrations in cell culture supernates, serum, plasma, urine, and other biological fluids. As a result of the donation of one electron, superoxide anion radicals o2 are produced in vivo, which are closely linked with several diseases in humans. This method may provide researchers in the food, nutrition, and medical fields an easy to use protocol to evaluate free radical scavenging. This package insert must be read in its entirety before using this product. Calculations the data were subjected to routine statistical analysis. Comparative study of abts radical scavenging activity and. Chrysin, rutin and quercetin were run to explore the effect of. The method dpph is widely used for measurement of free radical scavenging ability of antioxidants perezjimenez and sauracalixto, 2008.
Total antioxidant activity, metal chelation, radical scavenging dpph effects and reducing power as well as activities destructive to active oxygen species such as the superoxide anion radical, hydroxyl radical, and hydrogen peroxide are widely used for this purpose shimada et al. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal plants rozina parul1, sukalayan kumar kundu 2 and pijush saha2 1. Principle of dpph radical scavenging capacity assay. Pdf antioxidant activity by dpph radical scavenging. No extensive report on the presence, and free radical scavenging activity of basic metabolites from.
The compound sodium nitroprusside is known to decompose in aqueous solution at physiological ph 7. Although nac possesses its own free radical scavenging activity, its primary function in vivo is to supply cysteine necessary for glutathione synthesis and replenishment. Dpph free radical scavenging activity according to the method of pan et al. Materials and methods borage seeds were obtained from bioriginal food and science co. The assay is based on the reduction of mo vi to mo v by the extract and. The antioxidant activity using the dpph 1, 1diphenyl2picrylhydrazyl assay was assessed by the method of blois8. Department of agriculture, arkansas childrens nutrition center, 1120 marshall street. Albizia lebbeck, antioxidant activity, ethanolic root extract, dpph, nitric oxide. Antiradical scavenging activity was tested by the dpph model table 5. Antioxidant activity was determined by dpph radical scavenging and reducing power assays. Measuring antioxidant capacity using the orac and tosc. Measurement of superoxide anion scavenging activity was performed based on the method described by winterbourne et al 186.
All the extracts showed different levels of dpph radical scavenging activity over the range of 210 mgml concentration and the ec50 value of aqkf, mkf, ackf, ckf and pekf was found to be 4. In vitro antioxidant activity of coumarin compounds by. Evaluation of free radical scavenging activity of various. Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled mediterranean herbs and aromatic plants. In this study antioxidant activity was performed by dpph 1, 1diphenyl2picryl hydrazyl radical scavenging method for different extracts of aerial parts like leaves and flowers of ageratum.
The solutions were drawn into glass capillaries, sealed and measured using an esp300 instrument bruker spectrospin, karlsruhe, germany equipped with a high sensitivity tm110 xband cavity. The scavenging coefficient depends on the characteristics of the pollutant e. To compare the antioxdant activity we used abts 2,2azinobis 3ethylbenzothiazoline6sulfonic acid diammonium radical scavenging assay which is applicable for both lipophilic and hydrophilic antioxidants. Anticholinesterase, antioxidant and nitric oxide scavenging. Antioxidant activity by dpph assay of potential solutions to be. Free radical scavenging activity and lipoxygenase inhibition.
With contributions from worldclass experts in the field, the text presents the general mechanisms underlying the various assessments, the types of. Antioxidant and free radical scavenging activity of. Application of free radical diphenylpicrylhydrazyl dpph. How to find antioxidant properties from selected plants. The scavenging ratio is composed of two parameters, precipitation intensity mmhr and a scavenging coefficient smmhr1. The crude aqueous extract of the plant contains the phenolics and flavonoids which are said to be the potent antioxidants 11. The deoxyribose degradation assay is widely used to evaluate the hydroxyl oh radical scavenging ability of food or medicines. Previous studies have highlighted their anticestodal, antibacterial, wound healing activities, and so forth. The free radical scavenger ability of antioxidants can be predicted from standard oneelectron. Free radical scavenging activities of watersoluble extracts from some natural sources. Invitro antioxidant and free radical scavenging activity of. The following free radical scavenging assays were carried out.
Novel fluorometric assay for hydroxyl radical scavenging capacity. Dpph free radical scavenging activity of the extracts of. Novel fluorometric assay for hydroxyl radical scavenging. The free radical scavenging activities of these compounds were tested by their ability to bleach the stable radical dpph. The dpph assay method depends on the reduction of purple dpph to a yellow colored diphenyl picrylhydrazine and the. Solvent effects and improvements in the deoxyribose. The 2,2diphenylpicrylhydrazyl dpph assay is widely used in plant biochemistry to evaluate the properties of plant constituents for scavenging free radicals. In order to assess the radical scavenging potential of the crude extract, fractions i, ii and the compounds isolated, the reactivity towards the stable free radical dpph was measured at 518 nm by measurement of absorbance decrease of the reaction mixture after 5 hourreaction time. Hydroxyl radical scavenging activity of 18 pure organic solvents the hydroxyl oh radical scavenging activities of 18 pure organic solvents were determined, based on the original deoxyribose method, as described in the former section.
Rapid assay for testing superoxide anion radical scavenging. The percentage of radical scavenging activity by the sample treatment was. Withanolides analysis and comprehensive metabolic profiling between 100% methanol extracts of in vitro and field grown root tissues was performed using high. The intensity of the color formed was measured spectroscopically at 412 nm against reagent blank. Science and technology, general antibacterial agents rites, ceremonies and celebrations antioxidants antioxidants nutrients biphenyl compound chlorinated solvents herbal. Antioxidant and free radical scavenging activity of spondias. Although nac possesses its own free radical scavenging activity, its primary function in the body is to supply cysteine necessary for glutathione synthesis and replenishment. In vitro nitric oxide scavenging activity of methanol. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements ronald l.
Relationships between free radical scavenging and antioxidant. Nacetylcysteine nac is a stable form of the nonessential amino acid lcysteine. When an overload of free radicals cannot be scavenged, their. The objective of this study was to compare the free radical scavenging activity of various compounds to their ability to inhibit lipid oxidation in foods. Determination of free radical scavenging activity of plant. Antioxidant and free radicalscavenging properties of. Deoxyribose assay was used to determine the hydroxyl radical scavenging activity in an aqueous medium. Superoxide anion radical scavenging so assay superoxide anion is a weak oxidant, it gives rise to generation of powerful and dangerous hydroxyl radicals invitro super oxide radical scavenging activity is measured by riboflavinnbt nitro blue tetrazolium reduction.
Increasing the concentration of the extracts did not result in an increase in the nitrite radical scavenging activity. Based on dpph and hydroxyl radical scavenging activity, tpl showed strong scavenging activity 91. They are produced either from normal metabolic activities or from environmental. Antioxidant activity by dpph assay of potential solutions to. Dpph radical scavenging activity of various leaf extracts of k. A new fenton assay for hydroxyl radical scavengers by. Free radical scavenging activities of solutions of the plant extracts and synthetic antioxidant substances used in the study prepared in methanol at concentrations of 50, 100 and 200. Department of pharmacy, gono bishwabidyalay, savar, dhaka 44, bangladesh. The maximum free radical scavenging activity and potency were interpolated from figure 1 to give results as shown in table 1. Antioxidant capacity and radical scavenging effect of. Research paper superoxide anion radical scavenging activities. A novel fluorometric method was developed and validated for hydroxyl radical scavenging capacity hosc estimation using fluorescein as the probe. In this study, ethanol and ethyl acetate extracts of p. Total phenol and total flavonoid content in extracts, which reason antioxidant and free radical scavenging activity, was.
Free radical scavenging and antibacterial activities of. In vitro antioxidant and free radical scavenging activity of. Numerous attempts have been made to relate the free radical scavenging capacity of compounds to their antioxidant activity in foods even though antioxidant activity is dependent on both physical and chemical properties. Does anybody have a protocol for superoxide ion scavenging. International research journal of pharmaceutical and applied. Herbs and pastures, superoxide anion radical, scavenging activity, esr, hplc, altitudinal locations 1. Free radical scavenging effects of various extract of leaves of balanites aegyptiaca l. Superoxide, hydroxyl radical, lipid peroxidation, nitric oxide assay showed a comparable scavenging activity compared to its standard. The dpph radical himedia is stable due to the delocalization of a spare electron over the molecule, thus preventing dimer formation.
Free radical scavenging activity and antiproliferative. These compounds have been described as chainbreaking antioxidants acting through radical scavenging activity, that is related to their hydrogen or electron donating capacity and to the ability to delocalizestabilize the resulting phenoxyl radical within their structure. Determination of free radical scavenging activity from. The free radical scavenging activity of all the extracts was evaluated by 1, 1diphenyl2picrylhydrazyl dpph according to the previously reported method by shen et al. What is the standard dpph free radical scavenging activity. Estimation of nitric oxide scavenging activity of extracts was based on the diazotization reaction. The extract showed total antioxidant activity with a trolox equivalent antioxidant concentration teac value of 0. Vasumathi, yamini, jyothi and njavara were used to study their total phenolic and flavonoid contents, in vitro antioxidant activities including total antioxidant activity, scavenging of nitric oxide and 1,1diphenyl2picrylhydrazyl dpph radical, reducing power and cytotoxic. The generation of pure hydroxyl radicals under the experimental conditions was evaluated and. The first is the oxygen radical absorbance capacity orac assay, which measures the decrease in fluorescence decay caused by antioxidants, and the second is the total oxyradical scavenging capacity tosc assay, which measures the decrease in ethylene gas production caused by the inhibition of the thermal hydrolysis of abap 2,2. In vitro antioxidant capacity and free radical scavenging evaluation. In vitro antioxidant analysis and the dna damage protective activity.
These results suggest that tpl had the highest radical. Free radicals, formed in the course of normal cellular respiration and metabolism, and more abundantly under the influence of certain environmental chemicals and sunlight, have been inculpated in various types of tissue damage, particularly those involved in atherosclerosis, the aging process, and the development of cancers. Radical scavenging and antioxidant activity of ethanolic. Methanolic extracts of rice bran from four varieties. The scavenging activity percentage aa% was determined according to mensor et al. Phytochemical analysis and radical scavenging profile of. Free radical scavenging activity of ethanolic extracts from. Among them, the 2,2diphenyl1picrylhydrazyl dpph spectrophotometric method is one of the most widely applied and is appreciated for its reliability. Once formed, free radicals can start a chain reaction leading to formation of more free radicals.
Phytochemical analysis and free radical scavenging. As positive controls, epicatechin and lascorbic acid were also examined for dpph radical scavenging activity. Superoxide anion radical scavenging activity biology essay. Free radical scavenging activity, total phenolic content.
The dpph assay provides an easy and rapid way to evaluate potential antioxidants. Since the maximum absorption wavelength of the radical was observed at 750 nm, the absorbance of the extracts and standard reactions were measured at 750 nm, instead of 734. Invitro antioxidant and free radical scavenging activity. A modified version of the thiobarbituric acid reactive substances tbars assay was used to assess the. This unique formula also contains molybdenum, an essential trace element that functions as a cofactor in many biochemical reactions, and selenium, another trace mineral that. Among them, thyme and oregano exhibited the highest antioxidant activity, with i dpph values of 98.
The free radical scavenging activity of the ethanolic extracts was carried out based on a method developed by re et al. The extract was examined for different reactive oxygen species ros scavenging activities including hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and. The ic 50 values for scavenging of free radicals were 112. Antioxidant capacity and radical scavenging effect of polyphenol. Cysteine is a sulfurcontaining amino acid that acts as a stabilizer for the formation of protein structures. Free radical scavenging and total antioxidant capacity of. The antioxidant activity increased with an increase in concentration of the extracts reaching a plateau. Evaluation of nitrite radical scavenging properties of. The scavenging or inhibiting activity, expressed as mole hydroxyl radical scavenged or reduced per mole of test agent, was calculated as follows. In ferric reducing assay the absorbance were found to increase with increase in concentration of the extract revealing reducing power. Introduction a part of the oxygen taken into living cells is changed to several harmful reactive oxygen species and free radicals.
The dpph radical showed maximum inhibition of aqueous extract at 67. Intracellular ros protection efficiency and free radical. The anticholinesterase activity ic50 was observed at the concentrations of 2. The % hydroxyl radical scavenging activity of test sample was determined accordingly in comparison with negative control. International research journal of pharmaceutical and. The radical scavenging activity of different concentrations of curcumin was followed for 30 min by esr. The scavenging activity of different extracts of b. The method is based on the spectrophotometric measurement of the dpph concentration change resulting from the reaction with an antioxidant. Mallotus philippinensis is an important source of molecules with strong antioxidant activity widely used medicinal plant. The antioxidant activity of the memq was evaluated by the. Radical scavenging effects of different veronica species. Free radical scavenging activity from different extracts of. In the dpph free radical scavenging activity, three coumarin compounds i, ii and iii were evaluated for their free radical scavenging activity with ascorbic acid as standard compound. Measurement of antioxidant activity and capacity offers a muchneeded resource for assessing the antioxidant potential of food and includes proven approaches for creating healthy food products.
The samples were reacted with the stable dpph radical in an ethanol solution. Nitric oxide scavenging activity can be estimated by the use of griess iliosvoy reaction garrat, 1964. One of the quick methods to evaluate antioxidant activity is the scavenging activity on dpph, a stable free radical and widely used index. The antioxidant activity of the aerial part extract of m. Dpph inhibition in mpe was determined by using the protocol of brandwilliams et al. Hydroxy radical and dpph scavenging activity of crude. Protocol for life balance nac nacetylcysteine 600 mg. The nitric oxide scavenging activity, superoxide scavenging activity and hydroxyl radical scavenging activity was also concentration dependent with ic 50 value being 254. Antioxidant activities of indigofera cassioides rottl.
Jan 15, 2010 polygonumcuspidatum is widely used as a medicinal herb in asia. Free radical scavenging effect of various extracts of. Free radical scavenging activity of crude extracts and 4. Scavenging activity on dpph radical quantitative fig. The addition of antioxidants to the preformed radical cation, reduces it abts depending on the antioxidant activity and the concentration of the antioxidant. The results showed that six of eight mushroom polysaccharide extracts had superoxide and hydroxyl radical scavenging activities. Free radicalscavenging capacity, antioxidant activity and.
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